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December 12, 2013
To assist producers and their veterinarians in the management, control and potential elimination of porcine epidemic diarrhea virus (PEDV), the National Pork Board funded key research projects to better understand the disease. In order to provide timely information to producers from those projects, the objectives and initial updates will be periodically reported.
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The updates from the proposals represent interim information only and are not intended to be final reports. The final and formal reports will be provided at the end of the terms of the projects and then posted online at www.pork.org.
The information in these updates is intended to inform stakeholders of progress, but are not intended to be the final outcome or recommendation. For further information, please contact Lisa Becton, DVM, pork checkoff’s director of swine health information and research, at [email protected].
Project title, organization, objectives (Click on respective link to read full interim reports.) follow.
(University of Minnesota)
Determine the environmental stability of PEDV:
Aim 1. To determine survival of PEDV in fresh feces that represents the risk posed by transport.
Aim 2: To determine survival of PEDV in slurry (old feces in the pit) that reflects the risk of manure spreading.
Aim 3. To study PEDV survival in drinking and recycled water (truck washes).
Aim 4. To study PEDV survival in animal feed.
(University of Minnesota)
1. Prevalence & incidence of PEDV contamination in transport vehicles at slaughter.
2. Determine incidence of infection in unrelated sites adjacent to known infected sites and identify site-level risk factors associated with infection.
Propagation of PEDV in tissue culture and development of standardized reference samples for use in diagnostic testing (The Ohio State University)
1. Development and validation of diagnostic testing for PEDV antigen and antibody detection.
2. Development of standardized reference samples that can be utilized by veterinary diagnostic laboratories (VDLs) for diagnostic test validation.
3. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance.
Oral fluid testing for cost-effective and efficient surveillance and control of PEDV in swine population (Iowa State University)
1. Determine if oral fluids can be sample matrix to detect PEDV and/or virus-specific antibody.
2. Evaluate the performance of PEDV diagnostics on oral fluid samples.
3. Assess the utility of oral fluid sampling and testing for PEDV monitoring on farm.
Evaluation of time and temperature sufficient to kill PEDV in swine feces on metal surfaces (Iowa State University)
The objective of this study is to investigate the combinations of time and temperature sufficient to kill porcine epidemic diarrhea virus (PEDV) in swine feces on the metal surface of a model trailer used to haul live pigs. The results of this study will inform producers and veterinarians about the adequacy of current sanitation procedures for live haul trailers and enable them to make better decisions about investments in truck sanitation.
Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of PEDV following inoculation of feeder pigs (Kansas State University)
Study of the basic pathogenesis and characterization of the virus: Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of porcine epidemic diarrhea virus (PEDV) following inoculation of feeder pigs will be investigated. In an attempt to expand diagnostic testing capabilities, multiple aliquots (samples) of all will be collected and shared with requesting laboratories.
Development and validation of isolation and diagnostic testing detection for PEDV (University of Minnesota)
1. Development and standardization of viral propagation techniques to produce virus for use in diagnostic testing (outcome to be publicly available to all VDLs for PEDV diagnostics).
2. Development of standardized reference samples that can be utilized by VDLs for diagnostic test validation.
3. Validation of the current polymerase chain reaction ( PCR) diagnostic tests.
Development and validation for diagnostic testing for antigen and antibody detection for PEDV (University of Minnesota)
1. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance.
2. Develop and validate oral fluid testing (antigen and antibody testing capabilities) to provide rapid method for sample collection and to support on-farm surveillance.
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