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PCR catalog drives readiness for emerging swine diseases

The catalog is being sent to veterinary diagnostic labs and has been posted on the SHIC website to be readily available.

By Paul Sundberg, Swine Health Information Center Executive Director
When porcine epidemic diarrhea virus hit the United States in 2013, we could not effectively test for it. The North American pork industry learned a lesson and did something about it. Diagnostic preparedness and readiness for possible new or emerging production diseases has been a focus of the Swine Health Information Center since its 2015 inception.

The recent publication of the Polymerase Chain Reaction Assay Catalog for diagnostic laboratories demonstrates how far the pork industry has advanced in ability to test for emerging diseases.

Early detection is critical to early response. The catalog provides diagnosticians at our veterinary diagnostic labs, who are working every day with swine health case submissions, pertinent information about the 17 new PCR tests developed, including contact information of the experts for questions about availability and use. Additionally, the catalog summarizes the research behind the test development and covers technical background information including sample types and analytical and diagnostic sensitivity and specificity.

PCR Assay Catalog content

• Porcine Cytomegalovirus Real-Time PCR Validation for Diagnostic Use
• Development of Rapid Diagnostic Capability for Encephalomyocarditis Virus
• Detection and Differentiation of Influenza Types A, B, C and D in Swine
• Multiplex Real-Time RT-PCR Assay for Simultaneous Detection and Differentiation of Influenza C, D and B Viruses
• Implementation of real-time RT-PCR to Detect Porcine Hepatitis E
• Development and Validation of a Real-Time PCR to Detect Porcine Torovirus
• Swine Disease Matrix Antigen Detection Research Pathogen 1: Nipah Virus
• Development of a TaqMan Quantitative RT-PCR test for Porcine Parainfluenza Virus 1
• Development of a Sensitive and Reliable Diagnostic Assay to Detect Atypical Porcine Pestivirus in Swine
• Detection and Differentiation of Porcine Corona Virus 3 from PCV2a, PCV2b and the Highly Prevalent PCV2d Mutant Strains
• Development of PCR-Based Diagnostic Assays for Japanese Encephalitis Virus Infection in Pigs
• Development of a Dry Room Temperature-Stable Real-Time RT-PCR Assay for the Detection of Porcine Hemagglutinating Encephalomyelitis Virus
• Validation of a Real-Time Reverse Transcription PCR Assay for Detection of Porcine Sapelovirus
• Validation of a Real-Time Reverse Transcription PCR Assay for Detection of Porcine Teschovirus
• Development and Evaluation of a Real-Time RT-PCR and a Field-Deployable RT-Insulated Isothermal PCR for the Detection of Seneca Valley Virus
• Detection and Differentiation of Seneca Valley Virus from Foot-and-Mouth Disease Virus
• Validation of a Real-Time Reverse Transcription PCR Assay for Detection of Porcine Kobuvirus in Porcine Diagnostic Samples

The catalog is being sent to veterinary diagnostic labs and has been posted on the SHIC website to be readily available.

From evaluating risks via the SHIC Swine Disease Matrix and assessing our current diagnostic needs to be able to quickly identify these pathogens, to funding the development of tests, SHIC has led the pork industry to an additional level of readiness which puts the U.S. industry on a different playing field than it was on prior to SHIC’s inception.

Funded by America’s pork producers to protect and enhance the health of the U.S. swine herd, SHIC focuses its efforts on prevention, preparedness and response. As a conduit of information and research, SHIC encourages sharing of its publications and research for the benefit of swine health. For more information, visit the SHIC website or contact Paul Sundberg.

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