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Feed additives can modulate swine gut microbiome in weaned pigs

National Pork Board Feeder pigs in a pen
Average daily gain of pigs fed the PepM_Pro diets between days 15 and 35 was greater compared to pigs fed the Con diets.

Weaned pigs have an immature gut and microbiome which can impact digestion and absorption of nutrients. Digestive enzyme activities are reduced and altered due to changes in diet composition, feed intake and/or the environment. Alterations in gastrointestinal tract structure associated with weaning include the shortening of villi and increased crypt depth. Taken together, these conditions increase the susceptibility of weaned pigs to diarrhea due to enteric pathogens and to microorganisms that flourish in the presence of available nutrients in the lower intestine.

Feed additives such as peptides, mannose oligosaccharides and exogenous proteases have demonstrated varying effectiveness in improving weaned pig growth performance and in reducing post-weaning diarrhea. Peptides, as bioactive compounds as well as providing amino acids, were previously identified to accelerate the maturation of the swine gut microbiome (Poudel et al., 2020). In other examples, by improving the digestibility and absorption of nutrients in the small intestine, feed additives can influence the communities of the gut microbiome.

In a trial at the South Dakota State University commercial wean-to-finish research facility (Poudel et al., in press), 44 pens of mixed sex weaned pigs were fed one of four diets across a phase-feeding program during the nursery period. The feed additives, consisting of a peptide-mix, MOS and protease, were included only in Phase II and Phase III diets in this study and were all products of the same company. A common Phase I diet was fed from days 1 to 7, Phase II treatment diets from days 8 to 21, Phase III treatment diets from days 22 to 35 and a common Phase IV diet from days 36 to 49.

Pens were assigned to receive:

  1. Con: standard corn-soybean meal based diets with distiller's dried grains with solubles formulated to meet or exceed recommendations of NRC (2012) with no added peptide-mix, MOS or protease. 
  2. PepM: Con plus peptide-mix with MOS.
  3. PepM_Pro: Con plus peptide-mix with MOS and protease
  4. PepM_Pro(90): Con plus peptide-mix with MOS and protease with dietary amino acids provided at 90% of recommended levels according to NRC (2012).

The peptide-mix and MOS blend was added to the diets at 0.3%. As in a previous study, bacterial composition was determined from fecal samples collected at the end of Phase II and Phase III from 10 randomly selected, but representative pigs based on performance, at each timepoint. Specifically, fecal bacterial profiles at the end of Phase II and Phase III were compared from pigs in the Con, PepM and PepM_Pro treatment groups.

The average daily gain of pigs fed the PepM_Pro diets between days 15 and 35 was greater compared to pigs fed the Con diets. No differences in average daily feed intake were noted throughout the trial period. Gain:feed was improved for pigs fed the PepM diets compared to pigs fed Con diets between days 15 to 35 and days 0 to 35. However, gain:feed was reduced when pigs previously fed PepM and PepM_Pro were fed the common Phase IV diet compared to pigs fed Con diets.

Taxonomic profiling of fecal bacteria found that only two taxa, namely Streptococcaceae (Firmicutes) and Bacteroidaceae (Bacteroidetes), were lower in abundance from pigs fed Phase III Con diets compared to pigs fed Phase III PepM_Pro diets. There were no other differences between samples collected at the same time. Operational taxonomic unit analysis identified differences for three individual bacterial species. Namely, compared to pigs fed Con diets:

  1. Pigs fed Phase III PepM_Pro diets had higher abundance of Ssd-00039 (predicted to be a strain of Streptococcus alactolyticus).
  2. Pigs fed Phase II PepM_Pro diets had lower abundance of Ssd-00928 (predicted to be a currently uncharacterized species of the genus Ruminococcus).
  3. Pigs fed Phase III PepM diets had lower abundance of Ssd-01079 (predicted to be a currently uncharacterized species of the Clostridia, an order affiliated to Firmicutes).

The combination of the feed additives peptide-mix, MOS and protease investigated in this study provided benefits to the performance of weaned pigs and these feed additives modulated the swine gut microbiome. Furthermore, the most abundant candidate bacterial species, Ssd-00039, identified in this investigation may provide further insight into the impact of changes to the swine gut environment on the health and growth performance of nursery pigs.     

References
Poudel, P., C.L. Levesque, R.S. Samuel, and B. St-Pierre. 2020. Dietary inclusion of Peptiva, a peptide-based feed additive, can accelerate the maturation of the fecal bacterial microbiome in weaned pigs. BMC Vet. Res. 16:1-13. DOI: 10.1186/12917-020-02282-x

Poudel, P., R.S. Samuel, C.L. Levesque, and B. St-Pierre. In press. Investigating the effects of peptide-based, MOS and protease feed additives on the growth performance and fecal microbial composition of weaned pigs. J. Anim. Sci. Biotechnol.

Poudel, P., Samuel, R., Levesque, C.L. and St-Pierre, B. (2022). Investigating the effects of peptide-based, MOS and protease feed additives on the growth performance and fecal microbial composition of weaned pigs. Journal of Animal Science and Biotechnology 13, 25.

Source: Ryan Samuel, Prakash Poudel, Crystal Levesque, and Benoit St-Pierre, who are solely responsible for the information provided, and wholly own the information. Informa Business Media and all its subsidiaries are not responsible for any of the content contained in this information asset.

TAGS: Feed Nutrition
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